Western blot detection methods each have their own advantages and disadvantages. Choosing the right one can be a difficult decision. The basic protocol of the western blot – also referred to as the ...

Reviewed by Michael Greenwood, M.Sc. Fluorescent western blotting is a powerful protein detection technique that offers numerous benefits in comparison to alternative chemiluminescent or chromogenic ...

Western blotting is one of the most ubiquitous procedures used in the life science research lab today. Commonly used to assess relative protein expression differences, accurate Western blot ...

Western blotting remains a cornerstone method for the detection and quantification of proteins from complex biological samples. This technique has evolved from its early qualitative roots to a more ...

The western blotting technique that began as a means of detecting the protein of interest in a complex sample that may contain numerous other proteins has been in practice for four decades. In recent ...

Aldrin V. Gomes, Ph.D. Ning Liu Ph.D. A closer examination of housekeeping and other normalization techniques. In recent years, the scientific community has questioned the reliability of Western ...

However, they are all methods that rely on the use of antibodies to detect and identify proteins within biological samples. They can be used to asses and identify the topographical distribution of ...

The western blot is a method for detecting the presence and size of specific proteins. Proteins are first separated according to size or other physical properties by gel electrophoresis, then ...

One lab technique that has been around since what feels like the dawn of time is Western blotting, and it doesn’t look like it's going away anytime soon. Utilized by scientists worldwide, the Western ...

Western blotting is a critical method for protein analysis, but traditional workflows can be time-intensive and complex. Common challenges, such as lengthy staining processes and transfer ...

Mariella is an assistant editor at The Scientist. She has a background in neuroscience, and her work has appeared in Drug Discovery News and Massive Science. View full profile. Learn about our ...

For January’s Method of the Month I have selected the method presented by Kezzia S. Jones and colleagues in their recent reports article. The method utilizes binding to a surrogate protein (named ...